Mahmoud Ismail

Mahmoud Ismail (PhD ,MSc, BSc)

Research Assistant at Shriners Hospitals for Children.

Extensive experience in molecular biology ,biotechnology and diagnosis .

Research assistant (November 2020-present)
Shiners’ hospital for children –Research center -Montreal (QC)
Responsibilities include but not limited:
 Designing, preparing, and performing tissue, cell, and molecular biology-based approaches
such as cell culture, RNA/DNA extraction, PCR, Quantitative real time(qRT-PCR),
immunoblotting, plastic/paraffin sectioning and staining , immunohistochemistry, µCT
scanning, three point bending test (3-PBT), vertebra compression test, and data analysis.
 Rodent surgery: ovariectomy, Rodded Immobilized Fracture surgery.
 Helping in Writing grants and manuscripts
 Managing and organizing the stocking and supplies in the lab.
Necropsy tech- Division of Pathology-Necropsy
Charles River Laboratories, (CAN)
Responsibilities include but not limited:
 Small and large animal, anatomy, handling, restraint.
 Independently perform specialized necropsy procedures that may include but not limited to
collection of tissues for special procedures, perfusion fixation, specialized dissection, blood
and cerebral spinal fluid collections.
 Serve as lead/primary technician and independently perform necropsy functions and generate
data on any type of study.
Research Assistant (July 2018 – March 2019)
University of Montréal, School of Social Work. (CAN)
Responsibilities:
 Recruitment of participants for a research project on the settlement experiences of Syrian
refugees in Montreal.
 Interviews with participants and interpretation from Arabic to English.
Researcher II (January 2016 – June 2018)
University of Alabama at Birmingham, Division of Cardiovascular Disease (USA)
Responsibilities include but not limited:
 Managing supplies and equipment in the laboratory, keeping detailed records, writing reports,
preparing, and delivering oral presentations.
 Mice handling and drugs injection,
 Cell line culture, PCR and qRT-PCR ,
 Protein, DNA and RNA extraction and isolation
 Cryosectionning,Immunohistochemistry,
 SDS-gel electrophoresis and protein western blot,
 DNA cloning, DNA-electroelution from agarose gel.

PhD, Cardiovascular Sciences (2014 – 2018)
University of Verona (Italy), Graduate School for Health and Life Sciences, Department of
Cardiac Surgery
Major: Cardioprotection against Ischemia Reperfusion Injury.
Thesis: HDAC inhibition protects cardiomyocytes by modulating expression of opioid
Receptor and inducing prosurvival autophagy by inhibiting class I HD