Hema Bissessur

My name is Hema Devi Bissessur and I am an Honor Advanced-Biotechnology graduate. As a dynamic and versatile individual, I am looking forward to imparting the knowledge that I have acquired throughout these years and contributing my share to your organization. Additionally, my recent experience as a tutor at Seneca college has further broadened my communication and active listening skills. A skillset that differentiates me against most candidates would be my fluency in English, French, Hindi coupled with four years of experience in customer service. Seneca college has given industry experience, even as an entry level candidate. I feel confident that I am perfect for this job because I am willing to learn more about your company and as a dynamic and versatile individual, I am looking forward to imparting the knowledge that I have acquired throughout these years and contributing my share to your organization. Furthermore, I can precisely operate lab equipment such as autoclave, centrifuge, water baths, incubators, microscope, HPLC, GC-FID, GC-MS and others. In terms of interpersonal skills, I can procure effective time-management, attention to detail, critical thinking, and problem-solving strategies, while still being able to work well independently or in a group. These are skills that I bring to the table as an entry-level candidate and I can promise that your company will not be disappointed upon choosing me.

Full-time Bilingual Customer Service Representative at Global Payments (Toronto, May 2021- Present)

• Trained to use software programs such as Sales Force, Intranet, Base24, MAS, MOSS 2, Portico, EZPOS, TMS, and others.
• Maintaining a world class call handling etiquette and protocol by taking inbound calls.
• Helping merchant to set up pin pads and terminals of various models; Ingenico, Veriphone, PAX, and Castles.
• Troubleshooting basic and advanced customer service issues expressed by merchants. 
• Balancing, and adjusting return limits for merchants.
• Assisting merchants with statements, report, and using the Merchant Portal Website.

Peer Tutor Position Learning Centre at Seneca College (Toronto, May 2023-April 2023)

• Completed tutor training via Tutor Lingo.
• Assisted 1st and 2nd year tutees with exercises and concepts related to coursework.
• Tutored over 15 different courses both hybrid and in person.
• Mastered applications such as Humanity, Zoom, and Teams.
• Utilized effective communication, active listening, and critical thinking to help students develop metacognitive strategies in learning.

Pharmaceutical & Food Microbiology:  Experience working with BSL 2 pathogens and infectious models, Aseptic sampling & staining (gram stain, simple stain, endospore staining), Dilutions, plating and microscopic analysis of bacterial colonies, Microbial testing using Biochemical and Immunological techniques, Food safety and quality inspection through HACCP & MFHPB-21 rules, Fungi & Mold analysis from dichotomous keys, Environmental monitoring for air & water quality (MAS, RODAC, sw_2023ab method, REC tests). Preparing and sterilizing Agar, Pour & Plating of Bacteria, Catalase & Oxidase tests. 

Molecular Biology: PCR/qPCR, Immunofluorescence, Western blot, SDS page, CRISPr Cas 9, RNA/DNA/Protein isolation from tissues/cells, Library sequencing using bioinformatics, Column and size-exclusion chromatography, Design PCR and DNA sequencing primers, DNA foot-printing, Agarose Gel Electrophoresis, Size and map DNA fragments, Insertion of foreign DNA into expression vectors, Restriction digest, Gel extraction, Recombinant DNA transformation into bacteria.

Tissue culture:  Flow cytometry, Mammalian cell culture &  Cryopreservation, Aseptically growing monolayer cultures, Enumerate cells and set up experiments, Cell growth rates and plating efficiencies, Clone cells, Prepare cell lines from chicken embryos, Troubleshoot issues associated with maintaining healthy cell cultures, Clinical & Research Ethics.

Protein Biochemistry & Immunology: Antibody purification, ELISA, Separation, and quantification of mononuclear blood cells from whole blood, Immunoblot Assay, Small laboratory animal handling, Lateral flow assays, and rapid immunodiagnostics, Enzyme kinematics, Biuret, Lowry, and Bradford Assays.

Virology: Plaque assays, Generations of phage lysates, Maintenance of water baths and incubators, Proper handling and disposal of biohazards, Accurate & aseptic serial dilutions and calculations, Infection of E. coli with bacteriophage, Accurate calculation of viral titre based on plaque counts. 

Analytical Chemistry: Preparing, labeling and aliquoting stock solutions (buffers, reagents) according to standardized NIOSH protocols, Simple & Fractional distillations, Titrations, Instrumental calibration using external and internal standards, QA/ QC on-site (soil, air, water) testing for harmful metals & compounds, H’NMR spectrum, SCAN and SIM modes in mass spectral analysis, UV/VIS Spectroscopy, GC-MS, and LC-MS ( Electrospray), Beer-Lambert law in quantitative analysis, Cold Vapor AA Analysis of Hg in Air, (GC-FID),  IR Spectrophotometry, Computer Controlled Flame AAS, Acid Sample digestion, Matrix Interference. 

BIOINFORMATICS  & BIOPHARMACEUTICALS: Sequence Analysis, Phylogenetic, Structure & Gene Prediction, Expression Analysis, Heat maps using the R programming language, Rational Drug Design, Combinatorial chemistry methods and strategies, High throughput screening, Biopharmaceutical production in heterologous hosts, Recombinant protein production in mammalian culture. 

Apparatus: HPLC | Coulter Counter | Hemocytometer | Spectrophotometer | Flow cytometer | Biosafety Cabinets | Centrifuge | Autoclave | Micro-incinerator | Microscope | FT-IR | H’NMR |  GC-MS | SIE | GC-FID | IR spectroscopy | ICP-OES | Hydrometer | MP50 | Rotameter | ChemStation | Bio-Rad Digital Imaging System | Micropipettes | Burettes | Nano-Spectrophotometer | UV-VIS | Digital & Analogue Refractometer.